m6A methylation in the perinatal field

N6-methyladenosine (m6A), a major modification of messenger RNA (mRNA) and long non-coding RNA, plays critical roles in RNA metabolism and function [1]. Among the many types of RNA modification, m6A is the most frequent and abundant chemical post-transcriptional RNA modification [2]. Methylated RNA immunoprecipitation followed by sequencing (MeRIP-Seq) is a comprehensive assay to determine the presence of m6A [1]. Since the development of this assay, the functions of m6A have gradually become clearer m6A within coding mRNA are most abundantly observed in the vicinity of the stop codon, especially within the 3 UTR, and they have a consensus sequence of RRACH, where R is a purine and H is any base except for G [1]. m6A is involved in post-transcriptional regulation, especially in determining the stability and lifespan of mRNA [3].


Kosuke Taniguchi, Tomoko Kawai

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